The promoter is a DNA sequence that regulates gene expression in the upstream region of the gene and includes several major types, such as constitutive expression, tissue-specific expression, and inducible expression. Among them, the tissue-specific expression promoter drives the expression of the target gene at a specific tissue site or development stage of the plant, and can perform targeted genetic improvement on the plant traits.
Dr. Tao Yanbin from the Molecular Breeding Research Group of the Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, and his collaborators, under the guidance of researcher Xu Zengfu, first performed the expression level of the APETALA1 (JcAP1) gene in various tissues of Jatropha curcas. Analysis found that the gene was expressed in inflorescence buds, female flowers, and male flowers of Jatropha curcas, but almost none in other tissues; the expression level in the inflorescence buds was much higher than that in female flowers and male flowers. It was concluded that the JcAP1 gene is a flower-specific expression gene. Then, a 1.5 kb JcAP1 promoter fragment was further cloned and fused with the reporter gene GUS (JcAP1-GUS) to transform Arabidopsis thaliana. After promoter activity analysis, the JcAP1 promoter was found only in Arabidopsis inflorescence buds. It is a flower-specific promoter with expression activity in stamens and the -1313/-1057 segment of the promoter is essential for its activity in inflorescence buds. Further analysis of the promoter activity in JcAP1-GUS transgenic Jatropha shows that the JcAP1 promoter has high expression activity in seeds, in addition to high expression activity in Jatropha curcas buds. Thus, JcAP1 promoter from J. curcas itself can be used to drive the expression of functional genes in Jatropha sprouts and seeds.
The results of the relevant study were published in the international professional journal Planta. The topic is Isolation and characterization of the Jatropha curcas APETALA1 (JcAP1) promoter conferring preferential expression in inflorescence buds.
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