Beta-carotene is a natural pigment that belongs to the tetraterpene compound and has high pharmacological and nutritional value. Now it has been widely used in medicine, health care products, food additives and cosmetics and other industries. The construction of microbial strains for efficient synthesis of β-carotene has a good application prospect.
Li Qian, a master student in synthetic biology of natural products at the Wuhan Botanical Garden, Chinese Academy of Sciences, under the guidance of Professor Zhang Yansheng, reconstructed the biosynthetic pathway of beta-carotene from Saccharomyces cerevisiae. Carotene synthesis related genes crtYB (phytoene synthase and lycopene cyclase gene) and crtI (phytoene desaturase gene) were transferred into S. cerevisiae and these two genes were Codon optimization; simultaneous increase in yield of beta-carotene by overexpression of the key rate-limiting enzyme HMGR (3-hydroxy-3-methylglutaryl coenzyme A reductase) gene in the isopenm metabolic pathway A lot of, and no effect on the growth of Saccharomyces cerevisiae, and ultimately get Saccharomyces cerevisiae engineering strains with the ability to efficiently synthesize β-carotene.
This study provided a basis for the large-scale production of β-carotene by Saccharomyces cerevisiae, and also provided a theoretical basis for the biosynthesis and metabolic engineering of anthraquinones in Saccharomyces cerevisiae.
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